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In some case, the cell does not naturally produce Cas protein and is genetically engineered
to produce a Cas protein. As set forth elsewhere in this disclosure,
a DNA-binding domain can be engineered to bind
to virtually any desired sequence. Pat. Nos. 6,453,
242 and 6,794,136), one or more of the individual zinc fingers of a multi-finger
binding domain can bind to overlapping quadruplet subsites.

Pat. Nos. 8,586,526; 8,592,645; 8,399,218; 8,329,986; 7,888,121;
6,453,242; and 6,534,261 and U.S. See, also, U.S. Patent Publication Nos.

See, e.g., U.S. Pat. The disclosed methods and compositions include
fusion proteins comprising a DNA-binding domain (e.g., ZFP,
TALE, etc.) and a regulatory domain or cleavage (e.g., nuclease) domain (or
a cleavage half-domain), in which the DNA-binding domain, by binding to a sequence
in cellular chromatin in one or more plant genes, induces cleavage and targeted integration of one or
more exogenous sequences (including transgenes) into the
vicinity of the target sequence.